| Place | Bib | Lane | Name | Affiliation | Time |
| 1 | XH | 5 | Sheepshead | Sheepshead | 50.01 |
| 2 | XO | 8 | So River | So River | 50.88 |
| 3 | XY | 4 | Golding | Golding | 51.40 |
| 4 | XP | 6 | So Regional | So Regional | 51.94 |
| 5 | XE | 9 | Seton Keough | Seton Keough | 52.18 |
| 6 | XJ | 3 | Sherwood | Sherwood | 52.21 |
| 7 | XG | 7 | Shawnee | Shawnee | 53.90 |
| 8 | XD | 1 | Seneca | Seneca | 54.05 |
| 9 | XT | 2 | Sterling | Sterling | 57.14 |