| Place | Bib | Lane | Name | Affiliation | Time |
| 1 | PO | 7 | CB East | CB East | 49.11 |
| 2 | PB | 4 | Bowie | Bowie | 49.93 |
| 3 | PL | 2 | Cardozo | Cardozo | 49.94 |
| 4 | PH | 1 | CampusMagnet | CampusMagnet | 50.60 |
| 5 | PT | 9 | Cent York | Cent York | 51.84 |
| 6 | PR | 6 | CB West | CB West | 51.97 |
| 7 | PZ | 8 | Chesapeake | Chesapeake | 52.63 |
| 8 | PN | 5 | Central Pa | Central Pa | 53.32 |
| 9 | PY | 3 | C Hill East | C Hill East | 54.29 |