| Place | Bib | Lane | Name | Affiliation | Time |
| 1 | IK | 1 | Oberlin | Oberlin | 49.77 |
| 2 | II | 3 | NotreDame/Pa | NotreDame/Pa | 50.94 |
| 3 | IM | 5 | Octorara | Octorara | 51.46 |
| 4 | IJ | 2 | Oak Mills | Oak Mills | 52.36 |
| 5 | FS | 9 | Lampeter | Lampeter | 53.40 |
| 6 | IL | 4 | Ocean City | Ocean City | 53.45 |
| 7 | IN | 7 | Lady Mercy | Lady Mercy | 54.29 |
| 8 | IP | 8 | Packer | Packer | 54.96 |
| 9 | IG | 6 | Not Dame MA | Not Dame MA | 56.18 |