| Place | Bib | Lane | Name | Affiliation | Time |
| 1 | AA | 6 | LSU | LSU | 39.93 |
| 2 | AM | 8 | So Carolina | So Carolina | 40.50 |
| 3 | AL | 7 | TCU | TCU | 40.61 |
| 4 | AV | 9 | Texas | Texas | 41.06 |
| 5 | BJ | 5 | Del State | Del State | 41.89 |
| 6 | BG | 3 | Coastal | Coastal | 42.00 |
| 7 | BO | 2 | Wheel Jesuit | Wheel Jesuit | 42.41 |
| 8 | BN | 4 | The Hall | The Hall | 42.69 |
| 9 | BA | 1 | Ursinus | Ursinus | 44.11 |