| Place | Bib | Lane | Name | Affiliation | Time |
| 1 | QR | 9 | CH Flowers | CH Flowers | 43.48 |
| 2 | QY | 6 | Germantown | Germantown | 43.54 |
| 3 | NL | 2 | Barton | Barton | 44.33 |
| 4 | QO | 7 | Father Judge | Father Judge | 44.72 |
| 5 | QV | 4 | Gaithersburg | Gaithersburg | 44.78 |
| 6 | NF | 3 | Annapolis | Annapolis | 44.79 |
| 7 | QS | 8 | Fordham Prep | Fordham Prep | 44.87 |
| 8 | QU | 1 | Friendly | Friendly | 46.13 |
| 9 | QW | 5 | Garnet Vall | Garnet Vall | 49.31 |