| Place | Bib | Lane | Name | Affiliation | Time |
| 1 | BI | 9 | Pennbrook | Pennbrook | 53.44 |
| 2 | BH | 8 | ValForge | ValForge | 53.86 |
| 3 | BG | 7 | Tamanend | Tamanend | 54.36 |
| 4 | BE | 5 | Holicong | Holicong | 54.67 |
| 5 | BC | 3 | GreatVal | GreatVal | 55.23 |
| 6 | BD | 4 | Pennfield | Pennfield | 55.67 |
| 7 | BB | 2 | UpMerion | UpMerion | 56.03 |
| 8 | BF | 6 | Penndale | Penndale | 56.04 |
| 9 | BA | 1 | OJ Roberts | OJ Roberts | 56.07 |