| Place | Bib | Lane | Name | Affiliation | Time |
| 1 | 1 | 6 | William Wynne | Florida | 50.02 |
| 2 | 3 | 8 | Joe Greene | Albany | 50.53 |
| 3 | 4 | 9 | Adam Dailey | Nebraska | 50.91 |
| 4 | 5 | 5 | Dan Harmsen | Michigan | 51.67 |
| 5 | 6 | 4 | Patrick Lee | LSU | 52.32 |
| 6 | 75 | 1 | Brad McFadden | Temple | 53.98 |
| 7 | 74 | 2 | Mike Perri | Youngstown | 54.18 |
| 8 | 7 | 3 | Jordan Pitts | TCU | 54.24 |
| 9 | 2 | 7 | Johnny Dutch | So Carolina | 54.39 |