| Place | Bib Num | Name | Affiliation | Time | Heat |
| 1 | A | USA | NJ | 43.46 | 2 |
| 2 | B | US Hurdlers | VA | 44.39 | 2 |
| 1 | D | The Executives TC | NY | 45.10 | 1 |
| 2 | G | U.S. Elite | MD | 46.47 | 1 |
| 3 | N | Nike Central Park TC | NY | 48.04 | 1 |
| 4 | S | U.S. Elite | MD | 48.68 | 1 |
| 5 | H | Emerging Elite Athlete TC | NY | 48.75 | 1 |
| 3 | I | New Horizon T.C | NY | 48.96 | 2 |
| 6 | O | Greater Boston TC | MA | 50.44 | 1 |
| 7 | L | Bronx International Express | NY | 53.51 | 1 |
| 8 | Q | Drexel | PA | 59.01 | 1 |
| 9 | T | Drexel | PA | 59.84 | 1 |