| Place | Bib Number | Name | Affiliation | Time | Heat | Heat Place |
| 1 | B | HoustonElite | HoustonElite | 46.49 | 1 | 1 |
| 2 | C | Sprint Force | Sprint Force | 47.43 | 1 | 2 |
| 3 | A | Md Masters | Md Masters | 48.46 | 1 | 3 |
| 4 | H | Central Park TC | Columbia | 49.68 | 2 | 1 |
| 5 | I | PonyExp | PonyExp | 49.75 | 2 | 2 |
| 6 | F | M Velocity A | M Velocity A | 50.10 | 1 | 4 |
| 7 | G | Shore AC | Shore AC | 52.34 | 1 | 5 |
| 8 | D | Team Ohio | Team Ohio | 52.86 | 1 | 6 |
| 9 | E | 2nd Wind | 2nd Wind | 53.93 | 1 | 7 |
| 10 | J | M Velocity B | M Velocity B | 55.51 | 2 | 3 |